Applied Sciences > Agriculture > F-148

Research Title Utilization of cassava starch as a low-cost tissue culture medium components for rapid propagation of yam (Dioscorea alata L.) and cassava (Manihot esculenta Crantz)
Research Personnel Leader:
Prof. Villaluz Z. Acedo

Research Duration Start:
1 May 2012
30 April 2013
Research Location Leyte
Research Description In support to the yam and cassava industry, the Philippine Root Crops Research and Training Center (PhilRootcrops) is doing its best to develop improved varieties to address the needs of the stakeholders and at the same time developing strategies to fast-track planting material propagation. Non-conventional propagation methods such as tissue culture techniques have been utilized to make the newly-approved high-yielding root crop varieties available. With tissue culture techniques, mass propagation of clean planting materials for dissemination to stakeholders is possible. Protocols for rapid propagation of yam and cassava have been developed. The protocols are working well and that thousands of plantlets can be produced from one nodal segment in a year. However, just like in other economically important crops, the cost of tissue culture-derived plantlets is quite high considering the prices of the medium components and other laboratory supplies. In our desire to reduce production cost, it is fitting to explore the use of locally available low-cost alternatives for some culture components. This study would therefore explore the use of cassava starch as a substitute to the gelling agent (agar) without sacrificing other growth and development parameters in yam and cassava plantlets to be produced.
Research Objectives The project aims to determine the effects of substituting agar with cassava starch in the tissue culture propagation of yam and cassava in order to reduce production cost and to utilize locally available materials.
Research Beneficiary(ies) Tissue culture industry, researchers
Research Accoplishments In the process of refining the methodology for the right consistency of the yam for tissue culture.
Total Research Cost
Research Agencies Funding:
PhilRootcrops, VSU

Research Budget Breakdown Year:
Year Funded:
Total Cost
Date Released
Amount Released

Code F-148
KRA Code Rapid, inclusive, and sustained economic growth
Priority Thrust DOST
Create growth in the Countryside

Sector Applied Sciences
Actual Sector Agriculture
Related sectors Biotechnology, Food Technology
Entry revision: January 2021