Applied Sciences > Medicine > N-003

Research Title Development of Sterile Insect Technique for Dengue Mosquito Vector, Aedes aegypti using Gamma Irradiation
Research Personnel Leader:
Ms. Glenda B. Obra

Research Duration Start:
1 May 2012
30 April 2015
Research Location Quezon City
Research Description The incidence of dengue, a disease transmitted by mosquitoes of the genus Aedes, has increased dramatically around the world for the past few decades. Current estimates by WHO showed that there may be 50 million dengue infections worldwide every year. The disease is endemic in the Philippines among more than 100 countries in Africa, the Americas, the Eastern Mediterranean, South-east Asia and the Western Pacific. South-east Asia and the Western Pacific which are the most seriously affected. Currently, the primary method of controlling or preventing dengue virus transmission is to combat the vector mosquitoes (Aedes)using chemical methods. Although there have had some notable successes control has not been sustainable in the long term due to problems related to insecticide resistance, health and environmental concerns. Sterile insect technique (SIT), a species -specific, effective, and environmentally-friendly method of pest suppression or eradication has been successfully used against several insect species viz. screwworm flies, tephritid flies, other lepidopterous pests and in the elimination of tsetse fly. SIT involves the rearing, sterilization and release of insects rendered sterile by gamma radiation. A recent attempt to develop SIT against Aedes albopictus in Italy resulted in a significant decrease in the number of viable eggs in the release area compared to the control area (Bellini et al 2007). Hence, if Aedes aegypti, the primary vector of the disease, can be rendered sterile by gamma radiation without affecting the quality and competitiveness, then releases of sterile insects can be considered as an alternative method of control for this pest which can be integrated with other methods of control. The project will therefore be beneficial to the Filipinos by addressing the dengue problem brought by the mosquito vector.
Research Objectives (1) To establish rearing colony of A. aegypti in the laboratory; (2) To determine the radiosensitivity of A. aegypti to gamma radiation; (3) To determine the effect of irradiation on the adult survival/ longevity on A. aegypti; (4) To study the mating behavior of gamma irradiated A. aegypti in the laboratory.
Research Beneficiary(ies) Communities in urban and suburban areas, health sector, researchers
Research Accoplishments First trial, pupae consisting of both males and females were irradiated. Sterility study showed that irradiation of pupae at 25 Gy did not prevent adult female mosquitoes in laying eggs. Complete sterility was observed from female irradiated with 50Gy dose. Adult males that emerged from irradiated pupae were paired with virgin females in mylar cages to determine the effect of irradiaton on the sterility of male mosquitoes.
Total Research Cost
Research Agencies Funding:
Phil. Nuclear Research Institute

Research Budget Breakdown Year:
Year Funded:
Total Cost
Date Released
Amount Released

Code N-003
KRA Code Poverty Reduction and Empowerment of the Poor
Priority Thrust DOST
Pressing national problems

Sector Applied Sciences
Actual Sector Anti-dengue
Related sectors Healthcare, Nuclear Industry
Entry revision: January 2021