Applied Sciences > Medicine > D-131

Research Title Studies on the Structure and Biological Activity of the Phytochemicals from the Philippine Medicinal Tress "Ipil", "Handalamay", and "Lipang-Aso"
Research Personnel Leader:
Dr. Mylene M. Uy
Ms. Anita Rivera
Research Duration Start:
28 July 2008
28 July 2010
Research Location Mindanao State University-Iligan Institute of Technology, Iligan City
Research Description Plants hold a prominent position in the available sources of natural bioactive molecules. This is due to their sessile character which requires them to develop a large array of molecules to effectively respond to biotic and abiotic stimuli in situ. About 25% of the drugs prescribed worldwide come from plants, whereas 11% of the 252 drugs considered as basic and essential by the World Health Organization derive exlusively from plants. Moreover, traditional medicine have been in existence for years in most countries including the Philippines. This research is part of the research work being udertaken on the Philippine medicinal trees Intsia bijuga (Ipil), Pipturus arborescens (Handalamay), and Laportea aestuans (Lipang Aso)
Research Objectives The study aims to: (1) Isolate and purify the phytochemical components from the chloroform extracts of the various medicinal trees; (2) subject the purified components to cytotoxicity and antimicrobial assays, and (3) elucidate the structure of the purified components.
Research Beneficiary(ies) The study will significantly benefit the common people, most especially those living in the remote areas where access to mediocine is difficult or hampered. Furthermore, the results will eventually contribute to the rational and sustained exploration and conservation of the gene pool of the particular medicinal trees.
Research Accoplishments Purification of the respective crude chloroform fractions of “Ipil”, “Handalamay”, and “Lipang Aso” through silica gravity column chromatography employing appropriate solvent systems have yielded a number of sub fractions. Brine lethality tests of selected sub-fractions revealed some of them to have very remarkable cytotoxicitries against the brine shrimp A. salina. The highest cytotoxicity was exhibited by the “Ipil” sub-fractions IBC4.4 which has acute LC50 range value of 59.43-61.93 ppm followed by the “Handalamay” sub-fraction PAC4.3 (acute LC50 range=277.58-280-02 ppm) indicating that it only took six hours for these samples to kill 50% of the test animals. The next highest activities were showed by the “Ipil” sub-fraction IBS4.2 (chronic LC50 range value=146.94-149.42 ppm) followed by the “Handalamay” sub-fractions PAC4.3, PAC 4.4 and PAC4.8 (chronic LC50 range values=277.58-280.02, 412.77-414.83 and 415.17-417.23 ppm, respectively) signifying that the samples took twenty-four hours to kill 50% of the test animals. The HPLC purification of a “Lipang Aso” sub-fraction has lead to the isolation of 24-ethylcholesterol whose structure was determined from NMR spectral data. Almost all of the sub-fractions obtained exhibited radical-scavenging activity against the free-radical 2,2-diphenyl-1-picrylhydrazine (DPPH) indicating the samples to possess antioxidant potentials. Infrared spectroscopic measurements revealed the presence of the vinyl, carboxyl, nitro and ether functionalities in the samples. Phytochemical tests showed that saponins, flavonoids and alkaloids were common in the three medicinal plants. Tannins are found only in “Ipil” and “Handalamay” while terpenoids are present only in “Ipil” and “Lipang Aso”.
Total Research Cost ₱404,275
Research Agencies Funding:
Mindanao State University-Iligan Institute of Technology

Research Budget Breakdown Year:
Year Funded:
Total Cost
Date Released
Amount Released
2008 to 2010

Code D-131
KRA Code Rapid, inclusive, and sustained economic growth
Priority Thrust DOST
Develop Appropriate Tchnologies to Create Growth in the Countryside
Poverty Reduction and Empowerment of the Poor
Sector Applied Sciences
Actual Sector Medical Sciences
Related sectors Natural Products,Pharmaceutical Industry, Chemistry
Entry revision: January 2021